20.500.12556/RUL-145323 Bacillus subtilis PS-216 spores supplemented in broiler chicken drinking water reduce Campylobacter jejuni colonization and increase weight gain Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source of infection. To reduce the risk associated with C. jejuni transmission via poultry meat, effective interventions during poultry production are needed, and the use of probiotics is a promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. subtilis PS-216 strain demonstrated the best anti-Campylobacter activity against 15 C. jejuni isolates when examined using in vitro co-cultures. To evaluate the suitability of B. subtilis PS-216 for probiotic use, its susceptibility to eight clinically important antimicrobials and simulated gastric conditions was investigated. B. subtilis PS-216 was sensitive to all of the tested antibiotics. Although vegetative cells were sensitive to gastric conditions, B. subtilis PS-216 spores were highly resistant. We further evaluated the use of a B. subtilis PS-216 spore preparation (2.5 × 10$^6$ CFU/mL water) to prevent and/or reduce C. jejuni colonization in broiler chickens in vivo. Compared to the untreated group, significantly lower Campylobacter counts were detected in caeca of broilers continuously treated with B. subtilis PS-216 spores in their drinking water. Furthermore, broilers continuously treated with B. subtilis PS-216 spores showed improved weight gain, compared to the control group. Together, these results demonstrate the potential of B. subtilis PS-216 for use in poultry to reduce C. jejuni colonization and improve weight gain. Campylobacter jejuni Bacillus subtilis probiotics alternative to antibiotics spore-containing drinking water broiler chicken probiotiki inhibicija rasti sporogene bakterije perutnina true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-17 14:39:41 2023-04-17 14:39:45 2023-05-24 14:29:34 0000-00-00 00:00:00 2022 0 0 11 str. Vol. 13, art. 910616 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:579.67 1664-302X 10.3389/fmicb.2022.910616 115015683 fmicb-13-910616.pdf fmicb-13-910616.pdf 1 B29851300CE97B39D57D33529B998B8B e65a1ff06f9e885a38ab16cd68083b49732608fc89650c7a2dcd2d3c6905043d 56fa6e66-dd1d-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168153 https://www.frontiersin.org/articles/10.3389/fmicb.2022.910616/full 1 c680cbc6-dd1c-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168152 Biotehniška fakulteta 0 0 0