The role of endothelin B receptor in bone modelling during orthodontic tooth movement : a study on ET B knockout rats

Disha Ibrahimi, Saranda (avtor), Furlani, Borut (avtor), Drevenšek, Gorazd (avtor), Plut, Alja (avtor), Yanagisawa, M (avtor), Hudoklin, Samo (avtor), Prodan Žitnik, Irena (avtor), Marc, Janja (avtor), Drevenšek, Martina (avtor)

The endothelin system has an important role in bone modelling during orthodontic tooth movement (OTM); however, little is known about the involvement of endothelin B receptors (ETB) in this process. The aim of this study was to evaluate the role of ETB in bone modelling during OTM using ETB knockout rats (ETB-KO). Thirty-two male rats were divided into 4 groups (n = 8 per group): the ETB-KO appliance group, ETB-KO control group, wild type (ETB-WT) appliance group, and ETB-WT control group. The appliance consisted of a super-elastic closed-coil spring placed between the first and second left maxillary molar and the incisors. Tooth movement was measured on days 0 and 35, and maxillary alveolar bone volume, osteoblast, and osteoclast volume were determined histomorphometrically on day 35 of OTM. Next, we determined the serum endothelin 1 (ET-1) level and gene expression levels of the osteoclast activity marker cathepsin K and osteoblast activity markers osteocalcin and dentin matrix acidic phosphoprotein 1 (DMP1) on day 35. The ETB-KO appliance group showed significantly lower osteoblast activity, diminished alveolar bone volume and less OTM than the ETB-WT appliance group. Our results showed that ETB is involved in bone modelling in the late stage of OTM.

• Jezik: Angleški jezik
• Ključne besede: orthodontic tooth movement, endothelin system, bone modelling
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo ; MF - Medicinska fakulteta
• Različica publikacije: Objavljena publikacija
• Datum objave: 01.01.2020
• Leto izida: 2020
• Št. strani: 11 str.
• Številčenje: art. 14226, Vol. 10
• PID: 20.500.12556/RUL-141299
• UDK: 616.31
• ISSN pri članku: 2045-2322
• DOI: 10.1038/s41598-020-71159-8
• COBISS.SI-ID: 27363587
• Datum objave v RUL: 28.09.2022

Gradivo je del revije

• Naslov: Scientific reports
• Skrajšan naslov: Sci. rep.
• Založnik: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ortodontsko gibanje zob, endotelinski sistem, modeliranje kosti

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0293-2020
• Naslov: Parodontalna medicina