Whole-genome sequencing and annotation of the yeast Clavispora santaluciae reveals important insights about its adaptation to the vineyard environment

Franco-Duarte, Ricardo (avtor), Čadež, Neža (avtor), Rito, Teresa (avtor), Drumonde-Neves, João (avtor), Reyes Domínguez, Yazmid (avtor), Pais, Célia (avtor), Sousa, Maria João (avtor), Soares, Pedro (avtor)

Clavispora santaluciae was recently described as a novel non-Saccharomyces yeast species, isolated from grapes of Azores vineyards, a Portuguese archipelago with particular environmental conditions, and from Italian grapes infected with Drosophila suzukii. In the present work, the genome of five Clavispora santaluciae strains was sequenced, assembled, and annotated for the first time, using robust pipelines, and a combination of both long- and short-read sequencing platforms. Genome comparisons revealed specific differences between strains of Clavispora santaluciae reflecting their isolation in two separate ecological niches—Azorean and Italian vineyards—as well as mechanisms of adaptation to the intricate and arduous environmental features of the geographical location from which they were isolated. In particular, relevant differences were detected in the number of coding genes (shared and unique) and transposable elements, the amount and diversity of noncoding RNAs, and the enzymatic potential of each strain through the analysis of their CAZyome. A comparative study was also conducted between the Clavispora santaluciae genome and those of the remaining species of the Metschnikowiaceae family. Our phylogenetic and genomic analysis, comprising 126 yeast strains (alignment of 2362 common proteins) allowed the establishment of a robust phylogram of Metschnikowiaceae and detailed incongruencies to be clarified in the future.

• Jezik: Angleški jezik
• Ključne besede: genomics, phylogenomics, functional gene analysis, Metschnikowiaceae, Azores, wine yeasts, biotechnology, adaptation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 18 str.
• Številčenje: Vol. 8, iss. 1, art. 52
• PID: 20.500.12556/RUL-136749
• UDK: 579.25.065:582.28
• ISSN pri članku: 2309-608X
• DOI: 10.3390/jof8010052
• COBISS.SI-ID: 92329987
• Datum objave v RUL: 19.05.2022

Gradivo je del revije

• Naslov: Journal of Fungi
• Skrajšan naslov: J. Fungi
• Založnik: MDPI
• ISSN: 2309-608X
• COBISS.SI-ID: 525300761

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 05.01.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kvasovke, Clavispora santaluciae, filogenomika, sekvenciranje genoma