20.500.12556/RUL-136749 Whole-genome sequencing and annotation of the yeast Clavispora santaluciae reveals important insights about its adaptation to the vineyard environment Clavispora santaluciae was recently described as a novel non-Saccharomyces yeast species, isolated from grapes of Azores vineyards, a Portuguese archipelago with particular environmental conditions, and from Italian grapes infected with Drosophila suzukii. In the present work, the genome of five Clavispora santaluciae strains was sequenced, assembled, and annotated for the first time, using robust pipelines, and a combination of both long- and short-read sequencing platforms. Genome comparisons revealed specific differences between strains of Clavispora santaluciae reflecting their isolation in two separate ecological niches—Azorean and Italian vineyards—as well as mechanisms of adaptation to the intricate and arduous environmental features of the geographical location from which they were isolated. In particular, relevant differences were detected in the number of coding genes (shared and unique) and transposable elements, the amount and diversity of noncoding RNAs, and the enzymatic potential of each strain through the analysis of their CAZyome. A comparative study was also conducted between the Clavispora santaluciae genome and those of the remaining species of the Metschnikowiaceae family. Our phylogenetic and genomic analysis, comprising 126 yeast strains (alignment of 2362 common proteins) allowed the establishment of a robust phylogram of Metschnikowiaceae and detailed incongruencies to be clarified in the future. genomics phylogenomics functional gene analysis Metschnikowiaceae Azores wine yeasts biotechnology adaptation kvasovke Clavispora santaluciae filogenomika sekvenciranje genoma true false true Angleški jezik Slovenski jezik Članek v reviji 2022-05-19 10:53:04 2022-05-19 10:53:09 2022-09-15 04:14:17 0000-00-00 00:00:00 2022 0 0 18 str. iss. 1, art. 52 Vol. 8 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.25.065:582.28 2309-608X 10.3390/jof8010052 92329987 jof-08-00052.pdf jof-08-00052.pdf 1 D70B46D89552A419A79320CD063CB28A 21c9e07652ef32c360523f0f20c064beb69b08d07120b76488276dbbc63d5ec1 38f8f075-d751-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156518 https://www.mdpi.com/2309-608X/8/1/52 1 fd4cf29f-d750-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156517 Biotehniška fakulteta 0 0 0