izpis_h1_title_alt

Pulse duration dependent asymmetry in molecular transmembrane transport due to electroporation in H9c2 rat cardiac myoblast cells in vitro
ID Batista Napotnik, Tina (Avtor), ID Miklavčič, Damijan (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (3,56 MB)
MD5: 50AA34C063AFADDDCFC2209699F931A7
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/1420-3049/26/21/6571 Povezava se odpre v novem oknu

Izvleček
Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma membrane. Uptake of molecules after electroporation are the greatest at poles of cells facing electrodes and is often asymmetrical. However, asymmetry reported was inconsistent and inconclusive—in different reports it was either preferentially anodal or cathodal. We investigated the asymmetry of polar uptake of calcium ions after electroporation with electric pulses of different durations, as the orientation of elongated cells affects electroporation to a different extent when using electric pulses of different durations in the range of 100 ns to 100 µs. The results show that with 1, 10, and 100 µs pulses, the uptake of calcium ions is greater at the pole closer to the cathode than at the pole closer to the anode. With shorter 100 ns pulses, the asymmetry is not observed. A different extent of electroporation at different parts of elongated cells, such as muscle or cardiac cells, may have an impact on electroporation-based treatments such as drug delivery, pulse-field ablation, and gene electrotransfection.

Jezik:Angleški jezik
Ključne besede:electroporation, calcium uptake, electric field direction, nanosecond pulses
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FE - Fakulteta za elektrotehniko
Leto izida:2021
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Št. strani:22 str.
Številčenje:Vol. 26, iss. 21, art. 6571
PID:20.500.12556/RUL-136427 Povezava se odpre v novem oknu
UDK:602
ISSN pri članku:1420-3049
DOI:10.3390/molecules26216571 Povezava se odpre v novem oknu
COBISS.SI-ID:85047043 Povezava se odpre v novem oknu
Datum objave v RUL:03.05.2022
Število ogledov:89
Število prenosov:24
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
Objavi na:AddThis
AddThis uporablja piškotke, za katere potrebujemo vaše privoljenje.
Uredi privoljenje...

Gradivo je del revije

Naslov:Molecules
Skrajšan naslov:Molecules
Založnik:MDPI
ISSN:1420-3049
COBISS.SI-ID:18462981 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:01.11.2021

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:elektroporacija, vnos kalcija, smer električnega polja, nanosekundni pulzi

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Medtronic

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P2-0249
Naslov:Elektroporacija v biologiji, biotehnologiji in medicini

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:IP-0510

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj