20.500.12556/RUL-136427 Pulse duration dependent asymmetry in molecular transmembrane transport due to electroporation in H9c2 rat cardiac myoblast cells in vitro Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma membrane. Uptake of molecules after electroporation are the greatest at poles of cells facing electrodes and is often asymmetrical. However, asymmetry reported was inconsistent and inconclusive—in different reports it was either preferentially anodal or cathodal. We investigated the asymmetry of polar uptake of calcium ions after electroporation with electric pulses of different durations, as the orientation of elongated cells affects electroporation to a different extent when using electric pulses of different durations in the range of 100 ns to 100 µs. The results show that with 1, 10, and 100 µs pulses, the uptake of calcium ions is greater at the pole closer to the cathode than at the pole closer to the anode. With shorter 100 ns pulses, the asymmetry is not observed. A different extent of electroporation at different parts of elongated cells, such as muscle or cardiac cells, may have an impact on electroporation-based treatments such as drug delivery, pulse-field ablation, and gene electrotransfection. electroporation calcium uptake electric field direction nanosecond pulses elektroporacija vnos kalcija smer električnega polja nanosekundni pulzi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-05-03 15:06:26 2022-05-03 15:06:28 2022-09-15 04:13:52 0000-00-00 00:00:00 2021 0 0 22 str. iss. 21, art. 6571 Vol. 26 Nov. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602 1420-3049 10.3390/molecules26216571 85047043 molecules-26-06571.pdf molecules-26-06571.pdf 1 50AA34C063AFADDDCFC2209699F931A7 1e818ab0dd3b5c7a1beb260292c5a90782bc28091277fe35c2cfac1e9082fd3c f585159f-cae1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156074 https://www.mdpi.com/1420-3049/26/21/6571 1 c0f80cf9-cae1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156073 Fakulteta za elektrotehniko 0 0 0