A platform approach to protein encapsulates with controllable surface chemistry

Warner, Nina (avtor), Osojnik Črnivec, Ilja Gasan (avtor), Rana, Vijay Kumar (avtor), Cruz, Menandro (avtor), Scherman, Oren A. (avtor)

The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (D$_h$) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of D$_h$ is improved when measured at the spray dryer outlet temperature compared to room temperature (R$^2$ = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.

• Jezik: Angleški jezik
• Ključne besede: spray dry, protein, encapsulation, formulation, excipient, core-shell, EISA
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 20 str.
• Številčenje: Vol. 27, iss. 7, art. 2197
• PID: 20.500.12556/RUL-136115
• UDK: 66.047:577.112:615.014.6
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules27072197
• COBISS.SI-ID: 102605059
• Datum objave v RUL: 13.04.2022

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.04.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: sušenje z razprševanjem, proteini, kapsulacija, dostavni sistemi