20.500.12556/RUL-136115 A platform approach to protein encapsulates with controllable surface chemistry The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient’s preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (D$_h$) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of D$_h$ is improved when measured at the spray dryer outlet temperature compared to room temperature (R$^2$ = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying. spray dry protein encapsulation formulation excipient core-shell EISA sušenje z razprševanjem proteini kapsulacija dostavni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-13 13:17:25 2022-04-13 13:17:26 2022-09-15 04:13:25 0000-00-00 00:00:00 2022 0 0 20 str. iss. 7, art. 2197 Vol. 27 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.047:577.112:615.014.6 1420-3049 10.3390/molecules27072197 102605059 molecules-27-02197-v2-1.pdf molecules-27-02197-v2-1.pdf 1 4D6BF2ECB186E31D6E115360B9678552 6beeed2bcf0ed804a7ececd937d717a3e6b8c5505a790ed3e0526fa854849921 0cc0e1ee-c235-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155866 https://www.mdpi.com/1420-3049/27/7/2197 1 3fac659a-bb1b-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155588 Biotehniška fakulteta 0 0 0