TRIM28 selective nanobody reduces glioblastoma stem cell invasion

Porčnik, Andrej (avtor), Novak, Metka (avtor), Breznik, Barbara (avtor), Majc, Bernarda (avtor), Hrastar, Barbara (avtor), Šamec, Neja (avtor), Zottel, Alja (avtor), Jovchevska, Ivana (avtor), Vittori, Miloš (avtor), Rotter, Ana (avtor), Komel, Radovan (avtor), Lah Turnšek, Tamara (avtor)

Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool.

• Jezik: Angleški jezik
• Ključne besede: glioblastoma, nanobody, glioblastoma stem cells, cell invasion, transcription factor, TRIM28
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; BF - Biotehniška fakulteta ; FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 16 str.
• Številčenje: Vol. 26, iss. 17, art. 5141
• PID: 20.500.12556/RUL-135965
• UDK: 620.3:620.1/.2
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules26175141
• COBISS.SI-ID: 74227715
• Datum objave v RUL: 05.04.2022

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.09.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: glioblastom, TRIM28, nanoprotitelesa