TRIM28 selective nanobody reduces glioblastoma stem cell invasion

Porčnik, Andrej (author), Novak, Metka (author), Breznik, Barbara (author), Majc, Bernarda (author), Hrastar, Barbara (author), Šamec, Neja (author), Zottel, Alja (author), Jovchevska, Ivana (author), Vittori, Miloš (author), Rotter, Ana (author), Komel, Radovan (author), Lah Turnšek, Tamara (author)

Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool.

• Language: English
• Keywords: glioblastoma, nanobody, glioblastoma stem cells, cell invasion, transcription factor, TRIM28
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; BF - Biotechnical Faculty; FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 16 str.
• Numbering: Vol. 26, iss. 17, art. 5141
• PID: 20.500.12556/RUL-135965
• UDC: 620.3:620.1/.2
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules26175141
• COBISS.SI-ID: 74227715
• Publication date in RUL: 05.04.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.09.2021

Secondary language

• Language: Slovenian
• Keywords: glioblastom, TRIM28, nanoprotitelesa