Virome status of preclonal candidates of grapevine varieties (Vitis vinifera L.) from the Slovenian wine-growing region Primorska as determined by high-throughput sequencing

Miljanić, Vanja (avtor), Jakše, Jernej (avtor), Kunej, Urban (avtor), Rusjan, Denis (avtor), Škvarč, Andreja (avtor), Štajner, Nataša (avtor)

Diseases caused by viruses and virus-like organisms are one of the major problems in viticulture and grapevine marketing worldwide. Therefore, rapid and accurate diagnosis and identification is crucial. In this study, we used HTS of virus- and viroid-derived small RNAs to determine the virome status of Slovenian preclonal candidates of autochthonous and local grapevine varieties (Vitis vinifera L.). The method applied to the studied vines revealed the presence of nine viruses and two viroids. All viral entities were validated and more than 160 Sanger sequences were generated and deposited in NCBI. In addition, a complete description into the co-infections in each plant studied was obtained. No vine was found to be virus- and viroid-free, and no vine was found to be infected with only one virus or viroid, while the highest number of viral entities in a plant was eight.

• Jezik: Angleški jezik
• Ključne besede: Vitis vinifera L., preclonal candidates, HTS, viruses, viroids
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 11 str.
• Številčenje: Vol. 13, art. 830866
• PID: 20.500.12556/RUL-135073
• UDK: 634.8
• ISSN pri članku: 1664-302X
• DOI: 10.3389/fmicb.2022.830866
• COBISS.SI-ID: 98211843
• Datum objave v RUL: 21.02.2022

Gradivo je del revije

• Naslov: Frontiers in microbiology
• Skrajšan naslov: Front. microbiol.
• Založnik: Frontiers Research Foundation
• ISSN: 1664-302X
• COBISS.SI-ID: 4146296

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: vinska trta, sorte, klonska selekcija, predklonski kandidati, sekvenciranje, virusi, viroidi

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Public Scholarship, Development, Disability and Maintenance Fund of the Republic of Slovenia, Scholarship for nationals of Western Balkans to postgraduate study in Slovenia