20.500.12556/RUL-135073 Virome status of preclonal candidates of grapevine varieties (Vitis vinifera L.) from the Slovenian wine-growing region Primorska as determined by high-throughput sequencing Diseases caused by viruses and virus-like organisms are one of the major problems in viticulture and grapevine marketing worldwide. Therefore, rapid and accurate diagnosis and identification is crucial. In this study, we used HTS of virus- and viroid-derived small RNAs to determine the virome status of Slovenian preclonal candidates of autochthonous and local grapevine varieties (Vitis vinifera L.). The method applied to the studied vines revealed the presence of nine viruses and two viroids. All viral entities were validated and more than 160 Sanger sequences were generated and deposited in NCBI. In addition, a complete description into the co-infections in each plant studied was obtained. No vine was found to be virus- and viroid-free, and no vine was found to be infected with only one virus or viroid, while the highest number of viral entities in a plant was eight. Vitis vinifera L. preclonal candidates HTS viruses viroids vinska trta sorte klonska selekcija predklonski kandidati sekvenciranje virusi viroidi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-21 11:00:33 2022-02-21 11:00:39 2023-05-11 03:37:32 0000-00-00 00:00:00 2022 0 0 11 str. Vol. 13, art. 830866 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.8 1664-302X 10.3389/fmicb.2022.830866 98211843 RAZ_Miljanic_Vanja_2022.pdf RAZ_Miljanic_Vanja_2022.pdf 1 29A48B0DD16D9720997525B5B095E811 d81facb58693019573d51459e340aa6908b878afc546d7c2578f72d931cab0f8 9393f0dc-92fd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153931 https://www.frontiersin.org/articles/10.3389/fmicb.2022.830866/full 1 df80c9c6-92fc-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153930 Biotehniška fakulteta 0 0 0