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Extracellular vesicle isolation yields increased by low-temperature gaseous plasma treatment of polypropylene tubes
ID Resnik, Matic (Avtor), ID Kovač, Janez (Avtor), ID Štukelj, Roman (Avtor), ID Kralj-Iglič, Veronika (Avtor), ID Humpolíček, Petr (Avtor), ID Junkar, Ita (Avtor)

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MD5: C0B3CA4E77CDC19D11321774A601F513
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2073-4360/12/10/2363 Povezava se odpre v novem oknu

Izvleček
Novel Extracellular Vesicles (EVs) based diagnostic techniques are promising non-invasive procedures for early stage disease detection which are gaining importance in the medical field. EVs are cell derived particles found in body liquids, especially blood, from which they are isolated for further analysis. However, techniques for their isolation are not fully standardized and require further improvement. Herein modification of polypropylene (PP) tubes by cold Atmospheric Pressure Plasma Jet (APPJ) is suggested to minimize the EVs to surface binding and thus increase EVs isolation yields. The influence of gaseous plasma treatment on surface morphology was studied by Atomic Force Microscopy (AFM), changes in surface wettability by measuring the Water Contact Angle (WCA), while surface chemical changes were analyzed by X-Ray Photoelectron Spectroscopy (XPS). Moreover, PP tubes from different manufacturers were compared. The final isolation yields of EVs were evaluated by flow cytometry. The results of this study suggest that gaseous plasma treatment is an intriguing technique to uniformly alter surface properties of PP tubes and improve EVs isolation yields up to 42%.

Jezik:Angleški jezik
Ključne besede:atmospheric pressure plasma jets (APPJs), extracellular vesicles (EVs), nanostructures, polypropylene (PP)
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:ZF - Zdravstvena fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2020
Št. strani:13 str.
Številčenje:Vol. 12, iss. 10, art. 2363
PID:20.500.12556/RUL-134736 Povezava se odpre v novem oknu
UDK:533
ISSN pri članku:2073-4360
DOI:10.3390/polym12102363 Povezava se odpre v novem oknu
COBISS.SI-ID:46629379 Povezava se odpre v novem oknu
Datum objave v RUL:28.01.2022
Število ogledov:804
Število prenosov:164
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Polymers
Skrajšan naslov:Polymers
Založnik:Molecular Diversity Preservation International
ISSN:2073-4360
COBISS.SI-ID:517951257 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:15.10.2020

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:PR-06154

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