20.500.12556/RUL-134736 Extracellular vesicle isolation yields increased by low-temperature gaseous plasma treatment of polypropylene tubes Novel Extracellular Vesicles (EVs) based diagnostic techniques are promising non-invasive procedures for early stage disease detection which are gaining importance in the medical field. EVs are cell derived particles found in body liquids, especially blood, from which they are isolated for further analysis. However, techniques for their isolation are not fully standardized and require further improvement. Herein modification of polypropylene (PP) tubes by cold Atmospheric Pressure Plasma Jet (APPJ) is suggested to minimize the EVs to surface binding and thus increase EVs isolation yields. The influence of gaseous plasma treatment on surface morphology was studied by Atomic Force Microscopy (AFM), changes in surface wettability by measuring the Water Contact Angle (WCA), while surface chemical changes were analyzed by X-Ray Photoelectron Spectroscopy (XPS). Moreover, PP tubes from different manufacturers were compared. The final isolation yields of EVs were evaluated by flow cytometry. The results of this study suggest that gaseous plasma treatment is an intriguing technique to uniformly alter surface properties of PP tubes and improve EVs isolation yields up to 42%. atmospheric pressure plasma jets (APPJs) extracellular vesicles (EVs) nanostructures polypropylene (PP) true false true Angleški jezik Ni določen Članek v reviji 2022-01-28 08:40:22 2022-01-28 08:40:23 2022-09-14 04:09:38 0000-00-00 00:00:00 2020 0 0 13 str. iss. 10, art. 2363 Vol. 12 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 533 2073-4360 10.3390/polym12102363 46629379 RAZ_Resnik_Matic_2020.pdf RAZ_Resnik_Matic_2020.pdf 1 C0B3CA4E77CDC19D11321774A601F513 707b0760b0285af398a611b1864e1aeba65653bf4b7f3fd87a3aa29ff6870901 a8b0291b-800d-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153347 https://www.mdpi.com/2073-4360/12/10/2363 0 7194a520-800d-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153346 Zdravstvena fakulteta 0 0 0