Surfactin facilitates horizontal gene transfer in Bacillus subtilis

Danevčič, Tjaša (avtor), Dragoš, Anna (avtor), Špacapan, Mihael (avtor), Štefanič, Polonca (avtor), Dogša, Iztok (avtor), Mandić-Mulec, Ines (avtor)

Genetic competence for the uptake and integration of extracellular DNA is a key process in horizontal gene transfer (HGT), one of the most powerful forces driving the evolution of bacteria. In several species, development of genetic competence is coupled with cell lysis. Using Bacillus subtilis as a model bacterium, we studied the role of surfactin, a powerful biosurfactant and antimicrobial lipopeptide, in genetic transformation. We showed that surfactin itself promotes cell lysis and DNA release, thereby promoting HGT. These results, therefore, provide evidence for a fundamental mechanism involved in HGT and significantly increase our understanding of the spreading of antibiotic resistance genes and diversification of microbial communities in the environment.

• Jezik: Angleški jezik
• Ključne besede: quorum sensing, competence, surfactin, DNA exchange, extracellular DNA, cell lysis, horizontal gene transfer
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 8 str.
• Številčenje: Vol. 12, art. 657407
• PID: 20.500.12556/RUL-134068
• UDK: 579.22/.26:579.852.11
• ISSN pri članku: 1664-302X
• DOI: 10.3389/fmicb.2021.657407
• COBISS.SI-ID: 58756355
• Datum objave v RUL: 23.12.2021

Gradivo je del revije

• Naslov: Frontiers in microbiology
• Skrajšan naslov: Front. microbiol.
• Založnik: Frontiers Research Foundation
• ISSN: 1664-302X
• COBISS.SI-ID: 4146296

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Bacillus subtilis, zaznavanje kvoruma, horizontalni prenos genov, surfaktin