Electrotransfer of siRNA to silence enhanced green fluorescent protein in tumor mediated by a high intensity pulsed electromagnetic field

Kranjc Brezar, Simona (avtor), Kranjc, Matej (avtor), Čemažar, Maja (avtor), Buček, Simon (avtor), Serša, Gregor (avtor), Miklavčič, Damijan (avtor)

The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a treated tissue to a time-varying magnetic field. Due to the limited number of studies in the field of electroporation induced by HI-PEMF, we designed experiments to explore the feasibility of such a contactless delivery technique for the gene electrotransfer of nucleic acids in tissues in vivo. By using HI-PEMF for gene electrotransfer, we silenced enhanced green fluorescent protein (EGFP) with siRNA molecules against EGFP in B16F10-EGFP tumors. Six days after the transfer, the fluorescent tumor area decreased by up to 39% as determined by fluorescence imaging in vivo. In addition, the silencing of EGFP to the same extent was confirmed at the mRNA and protein level. The results obtained in the in vivo mouse model demonstrate the potential use of HI-PEMF-induced cell permeabilization for gene therapy and DNA vaccination. Further studies are thus warranted to improve the equipment, optimize the protocols for gene transfer and the HI-PEMF parameters, and demonstrate the effects of HI-PEMF on a broader range of different normal and tumor tissues.

• Jezik: Angleški jezik
• Ključne besede: pulsed electromagnetic field, siRNA, tumor, electroporation, gene therapy, high intensity pulsed electromagnetic field, electrotransfer, enhanced green fluorescent protein, mouse melanoma model
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko; ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 16 str.
• Številčenje: Vol. 8, iss. 1, art. 49
• PID: 20.500.12556/RUL-133258
• UDK: 602.621:616-006
• ISSN pri članku: 2076-393X
• DOI: 10.3390/vaccines8010049
• COBISS.SI-ID: 12843348
• Datum objave v RUL: 18.11.2021

Gradivo je del revije

• Naslov: Vaccines
• Skrajšan naslov: Vaccines
• Založnik: MDPI AG
• ISSN: 2076-393X
• COBISS.SI-ID: 520262937

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.03.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: pulzno elektromagnetno polje, siRNA, tumorji, elektroporacija, genska terapija