20.500.12556/RUL-133258 Electrotransfer of siRNA to silence enhanced green fluorescent protein in tumor mediated by a high intensity pulsed electromagnetic field The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of cell membrane permeability is similar to conventional electroporation, with the important difference of inducing an electric field non-invasively by exposing a treated tissue to a time-varying magnetic field. Due to the limited number of studies in the field of electroporation induced by HI-PEMF, we designed experiments to explore the feasibility of such a contactless delivery technique for the gene electrotransfer of nucleic acids in tissues in vivo. By using HI-PEMF for gene electrotransfer, we silenced enhanced green fluorescent protein (EGFP) with siRNA molecules against EGFP in B16F10-EGFP tumors. Six days after the transfer, the fluorescent tumor area decreased by up to 39% as determined by fluorescence imaging in vivo. In addition, the silencing of EGFP to the same extent was confirmed at the mRNA and protein level. The results obtained in the in vivo mouse model demonstrate the potential use of HI-PEMF-induced cell permeabilization for gene therapy and DNA vaccination. Further studies are thus warranted to improve the equipment, optimize the protocols for gene transfer and the HI-PEMF parameters, and demonstrate the effects of HI-PEMF on a broader range of different normal and tumor tissues. pulsed electromagnetic field siRNA tumor electroporation gene therapy high intensity pulsed electromagnetic field electrotransfer enhanced green fluorescent protein mouse melanoma model pulzno elektromagnetno polje siRNA tumorji elektroporacija genska terapija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-18 14:50:42 2021-11-18 14:50:42 2022-09-12 04:15:31 0000-00-00 00:00:00 2020 0 0 16 str. iss. 1, art. 49 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621:616-006 2076-393X 10.3390/vaccines8010049 12843348 RAZ_Kranjc_Brezar_Simona_2020.pdf RAZ_Kranjc_Brezar_Simona_2020.pdf 1 AD44B3E32837AAA02395EEB50A996C64 a65e06abd82f9d324aa73392b2137ef39ee23d46ac2a5440cf4a6075affb9b52 e6ba42f2-4876-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151163 https://www.mdpi.com/2076-393X/8/1/49 1 73916baa-4876-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151162 Fakulteta za elektrotehniko Zdravstvena fakulteta 0 0 0