Characterisation of lactoferrin isolated from acid whey using pilot-scale monolithic ion-exchange chromatography

Bogovič Matijašić, Bojana (avtor), Oberčkal, Jernej (avtor), Mohar Lorbeg, Petra (avtor), Paveljšek, Diana (avtor), Skale, Nina (avtor), Kolenc, Borut (avtor), Gruden, Špela (avtor), Poklar Ulrih, Nataša (avtor), Kete, Marko (avtor), Zupančič Justin, Maja (avtor)

The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM$^®$ monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m$^3$) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe$^{3+}$ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m$^3$/day capacity).

• Jezik: Angleški jezik
• Ključne besede: lactoferrin, acid whey, monolithic ion-exchange chromatography, E. coli O157:H7
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 19 str.
• Številčenje: Vol. 8, iss. 7, art. 804
• PID: 20.500.12556/RUL-132775
• UDK: 579.67:637.1:543.544.14
• ISSN pri članku: 2227-9717
• DOI: 10.3390/pr8070804
• COBISS.SI-ID: 23698947
• Datum objave v RUL: 03.11.2021

Gradivo je del revije

• Naslov: Processes
• Skrajšan naslov: Processes
• Založnik: MDPI
• ISSN: 2227-9717
• COBISS.SI-ID: 523353113

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 09.07.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrobiologija, mlekarstvo, kisla sirotka, laktoferin, protimikrobno delovanje, ionska izmenjevalna kromatografija

Projekti

• Financer: EC - European Commission
• Program financ.: LIFE
• Številka projekta: LIFE16 ENV/SI/000335
• Naslov: Reuse of waste acid whey for the extraction of bioactive proteins with high added value
• Akronim: LIFE for Acid Whey

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Support of research and development projects (TRL 3–6), S4—Networks for the transition to a circular economy, Biomass and alternative raw materials
• Naslov: Fractionation and processing of whey proteins and exploitation of the residue for the formation of new functional foods and food supplements
• Akronim: LAKTIKA