20.500.12556/RUL-132775 Characterisation of lactoferrin isolated from acid whey using pilot-scale monolithic ion-exchange chromatography The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM$^®$ monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m$^3$) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe$^{3+}$ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m$^3$/day capacity). lactoferrin acid whey monolithic ion-exchange chromatography E. coli O157:H7 mikrobiologija mlekarstvo kisla sirotka laktoferin protimikrobno delovanje ionska izmenjevalna kromatografija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-03 09:00:25 2021-11-03 09:00:25 2022-09-12 04:13:40 0000-00-00 00:00:00 2020 0 0 19 str. iss. 7, art. 804 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.67:637.1:543.544.14 2227-9717 10.3390/pr8070804 23698947 RAZ_Bogovic_Matijasic_Bojana_2020.pdf RAZ_Bogovic_Matijasic_Bojana_2020.pdf 1 0B72AEACADCC58EB42686E8E45FDACD5 9f765342403a658c1707a7adf9bf71489f541f406d3a60dcb3c2ab1d94df6a94 e9787ad2-5e47-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152164 https://www.mdpi.com/2227-9717/8/7/804 1 0fed857e-3c7c-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150470 Biotehniška fakulteta 0 0 0