Protein activity of the Fusarium fujikuroi rhodopsins CarO and OpsA and their relation to fungus-plant interaction

Adam, Alexander (avtor), Deimel, Stephan (avtor), Pardo-Medina, Javier (avtor), García-Martínez, Jorge (avtor), Konte, Tilen (avtor), Limón, M. Carmen (avtor), Avalos, Javier (avtor), Terpitz, Ulrich (avtor)

Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus.

• Jezik: Angleški jezik
• Ključne besede: fungal rhodopsins, protein activity, indole-3-acetic acid (IAA), CarO, OpsA, Fusarium fujikuroi, Oryza sativa, rice–plant infection, green light perception, bakanae, patch-clamp
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2018
• Št. strani: 20 str.
• Številčenje: Vol. 19, iss. 1, art. 215
• PID: 20.500.12556/RUL-131881
• UDK: 577
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms19010215
• COBISS.SI-ID: 33683161
• Datum objave v RUL: 05.10.2021

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 11.01.2018

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: glivični rodopsini, aktivnost beljakovin, indol-3-ocetna kislina (IAA)