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Protein activity of the Fusarium fujikuroi rhodopsins CarO and OpsA and their relation to fungus-plant interaction
ID
Adam, Alexander
(
Author
),
ID
Deimel, Stephan
(
Author
),
ID
Pardo-Medina, Javier
(
Author
),
ID
García-Martínez, Jorge
(
Author
),
ID
Konte, Tilen
(
Author
),
ID
Limón, M. Carmen
(
Author
),
ID
Avalos, Javier
(
Author
),
ID
Terpitz, Ulrich
(
Author
)
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http://www.mdpi.com/1422-0067/19/1/215
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Abstract
Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus.
Language:
English
Keywords:
fungal rhodopsins
,
protein activity
,
indole-3-acetic acid (IAA)
,
CarO
,
OpsA
,
Fusarium fujikuroi
,
Oryza sativa
,
rice–plant infection
,
green light perception
,
bakanae
,
patch-clamp
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2018
Number of pages:
20 str.
Numbering:
Vol. 19, iss. 1, art. 215
PID:
20.500.12556/RUL-131881
UDC:
577
ISSN on article:
1422-0067
DOI:
10.3390/ijms19010215
COBISS.SI-ID:
33683161
Publication date in RUL:
05.10.2021
Views:
746
Downloads:
158
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Record is a part of a journal
Title:
International journal of molecular sciences
Shortened title:
Int. j. mol. sci.
Publisher:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
11.01.2018
Secondary language
Language:
Slovenian
Keywords:
glivični rodopsini
,
aktivnost beljakovin
,
indol-3-ocetna kislina (IAA)
Projects
Funder:
Other - Other funder or multiple funders
Funding programme:
German Research Foundation
Project number:
TE 832/4-1
Funder:
Other - Other funder or multiple funders
Funding programme:
Spanish Government, Ministerio de Ciencia y Tecnología
Project number:
BIO2012-39716
Funder:
Other - Other funder or multiple funders
Funding programme:
Andalusian Government
Project number:
CTS-6638
Funder:
EC - European Commission
Funding programme:
European Regional Development Fund
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