Kompatibilnost entomopatogene glive Beauveria bassiana (Bals.-Criv.) Vuill. z antagonističnimi glivami iz rodu Trichoderma

Kenk, Jernej (avtor), Kos, Katarina (mentor)

Biotično varstvo rastlin v zadnjem obdobju dobiva vse večji pomen, saj predstavlja alternativo kemičnim fitofarmacevtskim sredstvom. V biotičnem varstvu se uporablja žive organizme, ki zatirajo škodljive organizme in obenem ne škodujejo ljudem. Mednje se uvršča tudi entomopatogeno glivo Beauveria bassiana in antagonistične glive iz rodu Trichoderma, ki so uporabljene v raziskavi. Izbrani so bili izolati dveh vrst antagonističnih gliv, in sicer T. asperellum in T. gamsii iz pripravkov Pannon Starter® Perfect Pro, Remedier® ter Trifender® Pro, ter entomopatogeno glivo B. bassiana iz pripravka Naturalis®. V raziskavi smo potrdili, da je temperatura 25 °C bolj optimalna za rast micelija vseh obravnavanih vrst, kot pa 15 °C. Ugotovili smo, da gliva T. gamsii dobro raste pri obeh temperaturah. Med glivami smo inhibicijo rasti opazili šele ob stiku micelijev entomopatogene in antagonistične glive. Izrazito inhibicijo rasti glive B. bassiana, s strani gliv iz rodu Trichoderma, smo ugotovili pri temperaturi 15 °C, a nasprotno pri temperaturi 25 °C, kjer je bila rast, do stika micelija gliv, nekoliko pospešena. Zaradi goste prepletenosti micelija ob stiku gliv nismo mogli potrditi inhibirane sporulacije ali parazitizma entomopatogene glive s strani antagonističnih gliv Trichoderma, vendar smo potencialen parazitizem opazili med glivo T. gamsii in B. bassiana pri temperaturi 15 °C.

• Jezik: Slovenski jezik
• Ključne besede: biotično varstvo, entomopatogena gliva, Beauveria bassiana, antagonistične glive, Trichoderma spp., mikoparazitizem, kompeticija
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Kraj izida: Ljubljana
• Založnik: [J. Kenk]
• Leto izida: 2021
• PID: 20.500.12556/RUL-130608
• UDK: 632.937:632.95:582.28:632.95.025.4(043.2)
• COBISS.SI-ID: 76423683
• Datum objave v RUL: 16.09.2021

KENK, Jernej, 2021, Kompatibilnost entomopatogene glive Beauveria bassiana (Bals.-Criv.) Vuill. z antagonističnimi glivami iz rodu Trichoderma [na spletu]. Magistrsko delo. Ljubljana : J. Kenk. [Dostopano 18 maj 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=130608

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Compatibilty of enhomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. with antagonistic fungivTrichoderma spp.

Izvleček

Biological pest control is becoming increasingly important as an alternative to pesticides used in agriculture. Living organisms and natural enemies are used in biological control to control pests or pathogens while being harmless to humans. The entomopathogenic fungus Beauvera bassiana and the antagonistic fungus Trichoderma spp. are biological agents used in our research. More specifically, two species of antagonistic fungi, T. asperellum and T. gamsii, isolated from the biostimulants Pannon Starter® Perfect Pro and Trifender® Pro and from a biological fungicide Remedier® were used. The entomopathogen B. bassiana was isolated from Naturalis®. Our experiment was conducted at two different temperatures, 15°C and 25°C. The results showed that temperature is an important factor for mycelial growth. At 25°C, mycelia of all fungi grew faster than at 15°C. Of all the fungi, T. gamsii was most successful at both temperatures. Strong inhibition of the growth of the fungus B. bassiana by Trichoderma fungi was observed at 15°C, while at 25°C the fungal growth of B. bassiana was accelerated until two mycelia came into contact. Growth inhibition between antagonistic and entomopathogenic fungi was observed only when different mycelia were in contact. The two associated mycelia were too intertwined to detect differences in sporulation or mycoparasitism by Trichoderma fungi. Potential parasitism was observed between T. gamsii and B. bassiana at 15°C.

• Ključne besede: biological control, entomopathogenic fungus, Beauveria bassiana, antagonistic fungi, Trichoderma spp., mycoparasitism, competition