Biological pest control is becoming increasingly important as an alternative to pesticides used in agriculture. Living organisms and natural enemies are used in biological control to control pests or pathogens while being harmless to humans. The entomopathogenic fungus Beauvera bassiana and the antagonistic fungus Trichoderma spp. are biological agents used in our research. More specifically, two species of antagonistic fungi, T. asperellum and T. gamsii, isolated from the biostimulants Pannon Starter® Perfect Pro and Trifender® Pro and from a biological fungicide Remedier® were used. The entomopathogen B. bassiana was isolated from Naturalis®. Our experiment was conducted at two different temperatures, 15°C and 25°C. The results showed that temperature is an important factor for mycelial growth. At 25°C, mycelia of all fungi grew faster than at 15°C. Of all the fungi, T. gamsii was most successful at both temperatures. Strong inhibition of the growth of the fungus B. bassiana by Trichoderma fungi was observed at 15°C, while at 25°C the fungal growth of B. bassiana was accelerated until two mycelia came into contact. Growth inhibition between antagonistic and entomopathogenic fungi was observed only when different mycelia were in contact. The two associated mycelia were too intertwined to detect differences in sporulation or mycoparasitism by Trichoderma fungi. Potential parasitism was observed between T. gamsii and B. bassiana at 15°C.
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