Bacteriophage delivery systems based on composite polyHIPE/nanocellulose hydrogel particles

Kopač, Tilen (avtor), Lisac, Ana (avtor), Mravljak, Rok (avtor), Ručigaj, Aleš (avtor), Krajnc, Matjaž (avtor), Podgornik, Aleš (avtor)

The role of bacteriophage therapy in medicine has recently regained an important place. Oral phage delivery for gastrointestinal treatment, transport through the stomach, and fast release in the duodenum is one of such applications. In this work, an efficient polyHIPE/hydrogel system for targeted delivery of bacteriophages with rapid release at the target site is presented. T7 bacteriophages were encapsulated in low crosslinked anionic nanocellulose-based hydrogels, which successfully protected phages at pH < 3.9 (stomach) and completely lost the hydrogel network at a pH above 3.9 (duodenum), allowing their release. Hydrogels with entrapped phages were crosslinked within highly porous spherical polyHIPE particles with an average diameter of 24 µm. PolyHIPE scaffold protects the hydrogels from mechanical stimuli during transport, preventing the collapse of the hydrogel structure and the unwanted phage release. On the other hand, small particle size, due to the large surface-to-volume ratio, enables rapid release at the target site. As a consequence, a fast zero-order release was achieved, providing improved patient compliance and reduced frequency of drug administration. The proposed system therefore exhibits significant potential for a targeted drug delivery in medicine and pharmacy.

• Jezik: Angleški jezik
• Ključne besede: TEMPO nanocellulose, T7 bacteriophage, encapsulation, drug diffusion, zero-order release
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 12 str.
• Številčenje: Vol. 13, iss. 16, art. 2648
• PID: 20.500.12556/RUL-129027
• UDK: 678:615:578.81
• ISSN pri članku: 2073-4360
• DOI: 10.3390/polym13162648
• COBISS.SI-ID: 73830403
• Datum objave v RUL: 24.08.2021

Gradivo je del revije

• Naslov: Polymers
• Skrajšan naslov: Polymers
• Založnik: Molecular Diversity Preservation International
• ISSN: 2073-4360
• COBISS.SI-ID: 517951257

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 09.08.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: TEMPO nanoceluloza, bakteriofag T7, inkapsulacija, difuzija učinkovin, sproščanje ničtega reda