Uromodulin and microRNAs in kidney transplantation - association with kidney graft function

Borštnar, Špela (author), Večerić-Haler, Željka (author), Boštjančič, Emanuela (author), Pipan Tkalec, Živa (author), Kovač, Damjan (author), Lindič, Jelka (author), Kojc, Nika (author)

Uromodulin and microRNAs (miRNAs) have recently been investigated as potential biomarkers for kidney graft associated pathology and outcome, with a special focus on biomarkers indicating specific disease processes and kidney graft survival. The study's aim was to determine whether expression of serum uromodulin concentration and selected miRNAs might be related to renal function in kidney transplant recipients (KTRs). The uromodulin concentration and expression of six selected miRNAs (miR-29c, miR-126, miR-146a, miR-150, miR-155, and miR-223) were determined in the serum of 100 KTRs with stable graft function and chronic kidney disease of all five stages. Kidney graft function was estimated with routine parameters (creatinine, urea, cystatin C, and Chronic Kidney Disease Epidemiology Collaboration study equations) and precisely measured using chromium-51 labelled ethylenediaminetetraacetic-acid clearance. The selected miRNAs were shown to be independent of kidney graft function, indicating their potential as biomarkers of associated kidney graft disease processes. In contrast, the serum uromodulin level depended entirely on kidney graft function and thus reflected functioning tubules rather than any specific kidney graft injury. However, decreased concentrations of serum uromodulin can be observed in the early course of tubulo interstitial injury, there by suggesting its useful role as an accurate, noninvasive biomarker of early (subclinical) kidney graft injury.

• Language: English
• Keywords: microRNA, uromodulin, kidney graft function, biomarker, kidney transplantation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 11 str.
• Numbering: Vol. 21, iss. 16, art. 5592
• PID: 20.500.12556/RUL-128786
• UDC: 616.6
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms21165592
• COBISS.SI-ID: 24550915
• Publication date in RUL: 29.07.2021

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 05.08.2020

Secondary language

• Language: Slovenian
• Keywords: mikroRNA, uromodulin, delovanje ledvičnega presadka, biološki označevalec, presaditev ledvic

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0323
• Name: Ledvične bolezni in nadomestna zdravljenja

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0054
• Name: Patologija in molekularna genetika