izpis_h1_title_alt

Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field
ID Dermol-Černe, Janja (Avtor), ID Batista Napotnik, Tina (Avtor), ID Reberšek, Matej (Avtor), ID Miklavčič, Damijan (Avtor)

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URLURL - Izvorni URL, za dostop obiščite https://www.nature.com/articles/s41598-020-65830-3 Povezava se odpre v novem oknu

Izvleček
In gene electrotransfer and cardiac ablation with irreversible electroporation, treated muscle cells are typically of elongated shape and their orientation may vary. Orientation of cells in electric field has been reported to affect electroporation, and hence electrodes placement and pulse parameters choice in treatments for achieving homogeneous effect in tissue is important. We investigated how cell orientation influences electroporation with respect to different pulse durations (ns to ms range), both experimentally and numerically. Experimentally detected electroporation (evaluated separately for cells parallel and perpendicular to electric field) via Ca$^2$$^+$ uptake in H9c2 and AC16 cardiomyocytes was numerically modeled using the asymptotic pore equation. Results showed that cell orientation affects electroporation extent: using short, nanosecond pulses, cells perpendicular to electric field are significantly more electroporated than parallel (up to 100-times more pores formed), and with long, millisecond pulses, cells parallel to electric field are more electroporated than perpendicular (up to 1000-times more pores formed). In the range of a few microseconds, cells of both orientations were electroporated to the same extent. Using pulses of a few microseconds lends itself as a new possible strategy in achieving homogeneous electroporation in tissue with elongated cells of different orientation (e.g. electroporation-based cardiac ablation).

Jezik:Angleški jezik
Ključne besede:electroporation, gene therapy, cardiac ablation
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FE - Fakulteta za elektrotehniko
Leto izida:2020
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Št. strani:17 str.
Številčenje:Vol. 10, art. 9149
UDK:602.621
ISSN pri članku:2045-2322
DOI:10.1038/s41598-020-65830-3 Povezava se odpre v novem oknu
COBISS.SI-ID:18364163 Povezava se odpre v novem oknu
Datum objave v RUL:22.07.2021
Število ogledov:283
Število prenosov:85
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Gradivo je del revije

Naslov:Scientific reports
Skrajšan naslov:Sci. rep.
Založnik:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:04.06.2020

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:elektroporacija, genska terapija, srčna ablacija

Gradivo je financirano iz projekta

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Medtronic PLC

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P2-0249
Naslov:Elektroporacija v biologiji, biotehnologiji in medicini

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:IP-0510

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:J2-9227
Naslov:Elektroporacijske terapije z novimi visokofrekvenčnimi elektroporacijskimi pulzi

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