20.500.12556/RUL-128666 Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field In gene electrotransfer and cardiac ablation with irreversible electroporation, treated muscle cells are typically of elongated shape and their orientation may vary. Orientation of cells in electric field has been reported to affect electroporation, and hence electrodes placement and pulse parameters choice in treatments for achieving homogeneous effect in tissue is important. We investigated how cell orientation influences electroporation with respect to different pulse durations (ns to ms range), both experimentally and numerically. Experimentally detected electroporation (evaluated separately for cells parallel and perpendicular to electric field) via Ca$^2$$^+$ uptake in H9c2 and AC16 cardiomyocytes was numerically modeled using the asymptotic pore equation. Results showed that cell orientation affects electroporation extent: using short, nanosecond pulses, cells perpendicular to electric field are significantly more electroporated than parallel (up to 100-times more pores formed), and with long, millisecond pulses, cells parallel to electric field are more electroporated than perpendicular (up to 1000-times more pores formed). In the range of a few microseconds, cells of both orientations were electroporated to the same extent. Using pulses of a few microseconds lends itself as a new possible strategy in achieving homogeneous electroporation in tissue with elongated cells of different orientation (e.g. electroporation-based cardiac ablation). electroporation gene therapy cardiac ablation elektroporacija genska terapija srčna ablacija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-22 11:44:45 2021-07-22 11:44:46 2022-09-07 04:16:30 0000-00-00 00:00:00 2020 0 0 17 str. art. 9149 Vol. 10 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621 2045-2322 10.1038/s41598-020-65830-3 18364163 RAZ_Dermol-Cerne_Janja_2020.pdf RAZ_Dermol-Cerne_Janja_2020.pdf 1 04B9DFFCAF7E14E0CA4C75FE196CC53B f4012ae52b4ed6318121307dfd054d5d4576e5d832e94bf518bb9ef59e440c4a d434f7c3-ead2-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145475 https://www.nature.com/articles/s41598-020-65830-3 1 4ab39aae-ead1-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145474 Fakulteta za elektrotehniko 0 0 0