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Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells
ID Prpar Mihevc, Sonja (Avtor), ID Kokondoska Grgič, Vesna (Avtor), ID Kopitar, Andreja Nataša (Avtor), ID Mohorič, Luka (Avtor), ID Majdič, Gregor (Avtor)

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Izvleček
Background: The ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulated; STIM1), followed by the induction of neuronal differentiation. Results: After 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases.

Jezik:Angleški jezik
Ključne besede:multipotent mesenchymal stromal cells, mesenchymal stem cells, dogs, differentiation, neurons, astrocytes
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
MF - Medicinska fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2020
Št. strani:12 str.
Številčenje:Vol. 16, art. 282
PID:20.500.12556/RUL-118719 Povezava se odpre v novem oknu
UDK:636.09:616.8
ISSN pri članku:1746-6148
DOI:10.1186/s12917-020-02493-2 Povezava se odpre v novem oknu
COBISS.SI-ID:25006595 Povezava se odpre v novem oknu
Datum objave v RUL:31.08.2020
Število ogledov:1125
Število prenosov:348
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Gradivo je del revije

Naslov:BMC veterinary research
Založnik:BioMed Central.
ISSN:1746-6148
COBISS.SI-ID:515674393 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:10.08.2020

Projekti

Financer:EC - European Commission
Program financ.:European Regional Development Fund

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0053
Naslov:Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

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