20.500.12556/RUL-118719 Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells Background: The ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulated; STIM1), followed by the induction of neuronal differentiation. Results: After 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases. multipotent mesenchymal stromal cells mesenchymal stem cells dogs differentiation neurons astrocytes true false true Angleški jezik Ni določen Članek v reviji 2020-08-31 14:12:24 2020-08-31 14:12:24 2022-08-31 03:49:34 0000-00-00 00:00:00 2020 0 0 12 str. art. 282 Vol. 16 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616.8 1746-6148 10.1186/s12917-020-02493-2 25006595 515674393 RAZ_Prpar_Mihevc_Sonja_2020.pdf RAZ_Prpar_Mihevc_Sonja_2020.pdf 1 BA254189CA1D0E4DCB26ECFD8FD86449 1f23d93913d67f3d18459e56fc3e6cf004dd0ba975dc72fa54eed1f351fbdc8e bc57badb-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141335 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02493-2 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=133113 Veterinarska fakulteta Medicinska fakulteta 0 0 0