Cell confinement reveals a branched-actin independent circuit for neutrophil polarity

Graziano, Brian R. (avtor), Town, Jason P. (avtor), Sitarska, Ewa (avtor), Nagy, Tamas L. (avtor), Fošnarič, Miha (avtor), Penič, Samo (avtor), Iglič, Aleš (avtor), Kralj-Iglič, Veronika (avtor), Gov, Nir S. (avtor), Diz-Muñoz, Alba (avtor), Weiner, Orion D. (avtor)

Migratory cells use distinct motility modes to navigate different microenvironments, but it is unclear whether these modes rely on the same core set of polarity components. To investigate this, we disrupted actin-related protein 2/3 (Arp2/3) and the WASP-family verprolin homologous protein (WAVE) complex, which assemble branched actin networks that are essential for neutrophil polarity and motility in standard adherent conditions. Surprisingly, confinement rescues polarity and movement of neutrophils lacking these components, revealing a processive bleb-based protrusion program that is mechanistically distinct from the branched actin-based protrusion program but shares some of the same core components and underlying molecular logic. We further find that the restriction of protrusion growth to one site does not always respond to membrane tension directly, as previously thought, but may rely on closely linked properties such as local membrane curvature. Our work reveals a hidden circuit for neutrophil polarity and indicates that cells have distinct molecular mechanisms for polarization that dominate in different microenvironments.

• Jezik: Angleški jezik
• Ključne besede: branched actin networks, neutrophil polarity, neutrophil motility, adherent conditions, membrane tension, cell confinement
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: Str. 1-34
• Številčenje: Vol. 17, iss. 10
• PID: 20.500.12556/RUL-113160
• UDK: 577
• ISSN pri članku: 1545-7885
• DOI: 10.1371/journal. pbio.3000457
• COBISS.SI-ID: 5728875
• Datum objave v RUL: 08.12.2019

Gradivo je del revije

• Naslov: PLoS biology
• Skrajšan naslov: PLoS biol.
• Založnik: Public Library of Science
• ISSN: 1545-7885
• COBISS.SI-ID: 2943764

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 08.12.2019