20.500.12556/RUL-113160 Cell confinement reveals a branched-actin independent circuit for neutrophil polarity Migratory cells use distinct motility modes to navigate different microenvironments, but it is unclear whether these modes rely on the same core set of polarity components. To investigate this, we disrupted actin-related protein 2/3 (Arp2/3) and the WASP-family verprolin homologous protein (WAVE) complex, which assemble branched actin networks that are essential for neutrophil polarity and motility in standard adherent conditions. Surprisingly, confinement rescues polarity and movement of neutrophils lacking these components, revealing a processive bleb-based protrusion program that is mechanistically distinct from the branched actin-based protrusion program but shares some of the same core components and underlying molecular logic. We further find that the restriction of protrusion growth to one site does not always respond to membrane tension directly, as previously thought, but may rely on closely linked properties such as local membrane curvature. Our work reveals a hidden circuit for neutrophil polarity and indicates that cells have distinct molecular mechanisms for polarization that dominate in different microenvironments. branched actin networks neutrophil polarity neutrophil motility adherent conditions membrane tension cell confinement true false false Angleški jezik Ni določen Članek v reviji 2019-12-08 13:20:24 2019-12-08 13:20:26 2022-08-25 03:55:03 0000-00-00 00:00:00 2019 0 0 Str. 1-34 iss. 10 Vol. 17 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1545-7885 10.1371/journal. pbio.3000457 5728875 2943764 RAZ_Graziano_Brian_R._2019.pdf RAZ_Graziano_Brian_R._2019.pdf 1 78ADD40021DBA648D9BD5D74C963F838 974d1b4cff0d1464688331fc0430cf256d99776e741f1531659a05013e9b9b7a 094a8e32-a1b8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=125192 https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000457 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=125191 Zdravstvena fakulteta 0 0 0