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Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline
Carrillo Rincon, Andres Felipe (Avtor), Magdevska, Vasilka (Avtor), Kranjc, Luka (Avtor), Fujs, Štefan (Avtor), Müller, Rolf (Avtor), Petković, Hrvoje (Avtor)

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URLURL - Izvorni URL, za dostop obiščite http://dx.doi.org/10.1007/s00253-018-8793-z Povezava se odpre v novem oknu

Izvleček
Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system.

Jezik:Angleški jezik
Ključne besede:Streptomyces rimosus, heterologous protein production, gene expression, gene tools, reporter system, phytase
Vrsta gradiva:Znanstveno delo (r2)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Št. strani:str. 2607-2620
Številčenje:Vol. 102, iss. 6
UDK:602.3+602.6+604.4:579.873.7
ISSN pri članku:0175-7598
DOI:10.1007/s00253-018-8793-z Povezava se odpre v novem oknu
COBISS.SI-ID:4879480 Povezava se odpre v novem oknu
Število ogledov:377
Število prenosov:284
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Gradivo je del revije

Naslov:Applied microbiology and biotechnology
Skrajšan naslov:Appl. microbiol. biotechnol.
Založnik:Springer
COBISS.SI-ID:5136135 Povezava se odpre v novem oknu

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:C3330-17-529038
Naslov:Raziskovalci-2.0-UL-BF-529038

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:streptomicete, Streptomyces rimosus, sekundarni metaboliti, heterologni proteini, izražanje genov, encimi, fitaze

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