Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline

Carrillo Rincon, Andres Felipe (avtor), Magdevska, Vasilka (avtor), Kranjc, Luka (avtor), Fujs, Štefan (avtor), Müller, Rolf (avtor), Petković, Hrvoje (avtor)

Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system.

• Jezik: Angleški jezik
• Ključne besede: Streptomyces rimosus, heterologous protein production, gene expression, gene tools, reporter system, phytase
• Vrsta gradiva: Znanstveno delo
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Recenzirani rokopis
• Št. strani: Str. 2607-2620
• Številčenje: Vol. 102, iss. 6
• PID: 20.500.12556/RUL-103067
• UDK: 602.3+602.6+604.4:579.873.7
• ISSN pri članku: 0175-7598
• DOI: 10.1007/s00253-018-8793-z
• COBISS.SI-ID: 4879480
• Datum objave v RUL: 13.09.2018

Gradivo je del revije

• Naslov: Applied microbiology and biotechnology
• Skrajšan naslov: Appl. microbiol. biotechnol.
• Založnik: Springer
• ISSN: 0175-7598
• COBISS.SI-ID: 5136135

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: streptomicete, Streptomyces rimosus, sekundarni metaboliti, heterologni proteini, izražanje genov, encimi, fitaze

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: C3330-17-529038
• Naslov: Raziskovalci-2.0-UL-BF-529038