20.500.12556/RUL-103067 Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system. Streptomyces rimosus heterologous protein production gene expression gene tools reporter system phytase streptomicete Streptomyces rimosus sekundarni metaboliti heterologni proteini izražanje genov encimi fitaze true false true Angleški jezik Slovenski jezik Znanstveno delo 2018-09-13 07:59:02 2018-09-13 09:54:20 2022-08-16 03:39:47 0000-00-00 00:00:00 0 0 0 Str. 2607-2620 iss. 6 Vol. 102 2018 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2019-02-07 602.3+602.6+604.4:579.873.7 0175-7598 10.1007/s00253-018-8793-z 4879480 5136135 Carrillo_et_al_2018_complete.pdf Carrillo_et_al_2018_complete.pdf 1 19E08577362A1A8691A575ABFC681FE5 ed189a7950bca302ea99e38771d2482fb1f51df2f0a041c12b77fea2af9ad36c b3be6db1-a1b5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=113455 http://dx.doi.org/10.1007/s00253-018-8793-z 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=113457 Biotehniška fakulteta 0 0 0