Mitochondrial biogenesis is complex process where expression of more than 1500 genes is necessary. The objective of this dissertation was developing expression profile of some important genes involved in regulation of mitochondrial biogenesis. NRF-1, NRF-2, PPARGC1A, TFAM, POLG, POLRMT, TWNIKLE are genes that influence on biogenesis and function of mitochondrion. For this research we chose six pig tissue (m. semispinalis capitis, m. semimembranosus, heart muscle, brain, spleen and liver). All pigs were male hybrid 1244 and krško polje breed. At the same time we have developed expression profile of alternatively spliced form of TFAM gene, which is important transcription factor in mitochondrial biogenesis. We used ENSEMBL and NCBI databases for collection of known pig data. For expression profile we used absolute quantification qPCR method. Analysis have shown that shorter, alternatively spliced, form of TFAM is expressed in all selected tissues. Shorter form of TFAM is missing HMG box 1 which is coded in exon 4 of the gene. From data obtained we conclude that this protein has impaired binding to mtDNA. With bioinformatics and statistical analysis we have shown that PPARGC1A is one of the most important proteins in first phases of mitochondrial biogenesis. With regulatory pathway analysis we have shown that PPARGC1A, NRF1 and SIRT1 are strongly connected in this process. For POLG, LONP1, OMA1 and TWNIKLE we could not prove that they were directly related to PPARGC1A, NRF11 and SIRT1.