'Candidatus Phytoplasma solani' is responsible for various different diseases on important crop plants including grapevine, where it causes bois noir disease. For the epidemiologic purposes we preformed a molecular characterisation of DNA samples from infected plants from two grapevine regions in Slovenia: Primorje (south-west) and Podravje (north-east). We analysed 124 different DNA samples from infected plants, 117 of them were from vinegrape, 5 from stinging nettle and 2 samples from carrot. Samples were taken in time period between 2003 and 2016. With RFLP analysis of the nested PCR product of conserved gene tuf, we divided the samples into two groups: tuf-a and tuf-b. Samples from the tuf-b group have been further divided into tuf-b1 and tuf-b2 subgroup (based on a difference in one nucleotide in a gene sequence). We have determined that both tuf-a and tuf-b are present in all investigated regions, with tuf-b being more abundant in both regions: 52 % in SW and 81 % in NE, but tuf-a being more present in SW (48 %) than NE (19 %). Subtype tuf-b1 was found in 62 % and tuf-b2 in 38 % out of 71 analysed tuf-b samples. Subtype tuf-b2 is with only one exeption present exclusively in NE region. Tuf-b2 is first detected in NE region in 2007 and is becoming more abundant each year.