The purpose of the thesis was to analyse the acceptance of Scrum methodology, which has become one of the leading agile methodologies, and to find out which were the key factors that influenced the acceptance. The analysis was conducted in Comtrade, which is one of the largest Slovenian software development companies. The First part (theoretical part) contains an introduction chapter, a detailed presentation of Scrum methodology and the presentation of theoretical models, on which practical (research) part of thesis is based. The Second part (practical part) consists of two chapters. In the first one the analysis of Scrum methodology usage in Comtrade is presented, where the analysis of acceptance of key activities and the analysis of key factors form the main part. The second chapter is devoted to the presentation of comparison between our research and research done by two German software development experts.