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Phytoplasma infection may affect morphology, regeneration and pyrethrin content in pyrethrum shoot culture
Ambrožič-Dolinšek, Jana (Avtor), Camloh, Marjana (Avtor), Žel, Jana (Avtor), Kovač, Maja (Avtor), Ravnikar, Maja (Avtor), Carraro, Luigi (Avtor), Petrovič, Nataša (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://dx.doi.org/10.1016/j.scienta.2007.11.013 Povezava se odpre v novem oknu

Izvleček
The aim of the work was to infect pyrethrum (Tanacetum cinerariifolium (Trevir.) Schulty-Bip.), an important secondary metabolite pyrethrin producing plant, with MA phytoplasma (16S rRNA group III, subgroup B) to initiate tissue culture of phytoplasma-infected plants, and to examine possible effects of infection on pyrethrin content, morphology, and regenerative capacity in shoot culture. Phytoplasma transmission was achieved bz using an insect vector (Euscelidius variegatus) making pyrethrum as a new host for phytoplasma. The infection significantly changed the morphology and regenerative capacity of shoots and roots. The infected shoots and roots were shorter and thinner and an increase in proliferation of both was observed. Phytoplasma infection significantly decreased pyrethrin content in shoots, as measured by HPLC. The possibility of long-term maintenance of phytoplasma was also studied. The presence of phytoplasma in shoots was confirmed periodically over 4 years by PCR assay. Possible physiological reasons for a balanced phytoplasma-host system are discussed.

Jezik:Angleški jezik
Ključne besede:Tanacetum cinerariifolium, pyrethrum, phytoplasma infection, shoot culture
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Leto izida:2008
Št. strani:str. 213-218
Številčenje:Vol. 116, no. 2
UDK:577
ISSN pri članku:0304-4238
COBISS.SI-ID:1842511 Povezava se odpre v novem oknu
Število ogledov:478
Število prenosov:200
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:Scientia horticulturae
Skrajšan naslov:Sci. hortic.
Založnik:Elsevier
ISSN:0304-4238
COBISS.SI-ID:5191687 Povezava se odpre v novem oknu

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