izpis_h1_title_alt

Metric characteristics of various methods for numerical density estimation in transmission light microscopy - a computer simulation
Kališnik, Miroslav (Avtor), Blejec, Andrej (Avtor), Pajer, Zdenka (Avtor), Majhenc, Janja (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.dlib.si/details/URN:NBN:SI:doc-G28BTM1E Novo okno

Izvleček
In the introduction the evolution of methods for numerical density estimation of particles is presented shortly. Three pairs of methods have been analysed and compared: (1) classical methods for particles counting in thin and thick sections, (2) original and modified differential counting methods and (3) physical and optical disector methods. Metric characteristics sush as accuracy, efficiency, robustness, and feasibility of methods have been estimated and compared. Logical, geometrical and mathematical analysis as well as computer simulations have been applied. In computer simulations a model of randomly distributed equal spheres with maximal contrast against sorrundings has been used. According to our computer simulation all methods give accurate results provided that the sample is representative and sufficiently large. However, there are differences in their efficiency, robustness and feasibility. Efficiency and robustness increase with increasing slice thickness in all three pairs of methods. Robustness is superior in both differential and both disector methods compared to both classical methods. Feasibility can be judged according to the additional equipment as well as to the histotechnical and counting procedures necessary for performing individual counting methods. However, it is evident that not all practical problems can efficiently be solved with models.

Jezik:Angleški jezik
Ključne besede:accuracy, efficiency, feasibility, light microscopy, numerical density, robustness, vertical resolution
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Leto izida:2001
Založnik:Društvo za stereologijo in kvantitativno analizo slike, Medicinska fakulteta
Št. strani:str. 15-25
Številčenje:Vol. 20, no. 1
UDK:621.38
ISSN pri članku:1580-3139
COBISS.SI-ID:836943 Povezava se odpre v novem oknu
Število ogledov:274
Število prenosov:463
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Gradivo je del revije

Naslov:Image analysis & stereology
Skrajšan naslov:Image anal. stereol.
Založnik:Društvo za stereologijo in kvantitativno analizo slike, Medicinska fakulteta
ISSN:1580-3139
COBISS.SI-ID:106479104 Novo okno

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