izpis_h1_title_alt

Isolation of endoxylanases from anaerobic bacterium Butyrivibrio sp. strain Mz5 is possible by anion exchange chromatography on CIM DEAE-8 monolythic column
Čepeljnik, Tadej (Author), Zorec, Maša (Author), Nekrep, Franc Viktor (Author), Marinšek-Logar, Romana (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:doc-B7BKQF8K This link opens in a new window

Abstract
The xylanolitically active enzymes are of great interest for the industry and as feed additives as well. Therefore an effort was made to search for the microbial strains capable to degrade xylan. One of the most active rumen bacteria was Butyrivibrio sp. strain Mz5 possessing multiple xylanolytic enzyme system. In the present work the procedure for the separation of two of them is outlined. This was successfully done by anion exchange chromatography followed by gel filtration. Using the CIM DEAE tube was the key isolating point that prevented othewise frequent aggregation of the proteins and speededup the procedure. The isolated enzymes were of 51 and 58 kDa.

Language:English
Keywords:mikrobiologija, bakterije, Butyrivibrio, endoksilanaze, izolacija, analitska kemija, kromatografija
Work type:Not categorized (r6)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Year:2002
Publisher:Slovensko kemijsko društvo
Number of pages:str. 401-408
Numbering:Letn. 49, št. 2
UDC:579
ISSN on article:1318-0207
COBISS.SI-ID:1251720 Link is opened in a new window
Views:835
Downloads:216
Metadata:XML RDF-CHPDL DC-XML DC-RDF
 
Average score:(0 votes)
Your score:Voting is allowed only to logged in users.
:
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Record is a part of a journal

Title:Acta chimica slovenica
Shortened title:Acta chim. slov.
Publisher:Slovensko kemijsko društvo, =Slovenian Chemical Society
ISSN:1318-0207
COBISS.SI-ID:14086149 This link opens in a new window

Secondary language

Language:Slovenian
Title:Izolacija endoksilanaz anaerobne bakterije Buyrivibrio sp. Sev Mz5 je mogoča z uporabo anionske izmenjevalne kromatografije s CIM DEAE-8 monolitnim modulom
Abstract:
Ksilanolitični encimi so pomembni v industriji celuloze in papirja ter prehranski industriji, uporabljamo pa jih tudi kot dodatek krmi ali hrani. Veliko truda je bilo vloženega v iskanje mikroorganizmov, ki so sposobni razgradnje ksilana. Ena izmed najbolj ksilanolitično aktivnih vampnih bakterij je Butyrivibrio sp. sev Mz5, ki sintetizira več različnih ksilanaz. V predstavljenem delu je opisan postopek izolacije dveh izmed njih z anionsko izmenjevalno kromtografijo in nadaljno gelsko filtracijo. Ključni izolacijski postopek je bila uporaba CIM DEAE monolitnega modula, s čimer smo se izognili drugače pogosti agregaciji proteinov in tudi pospešili celoten postopek. Izolorana encima imata molekulsko težo 51 in 58 kDa.


Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back