Isolation of endoxylanases from anaerobic bacterium Butyrivibrio sp. strain Mz5 is possible by anion exchange chromatography on CIM DEAE-8 monolythic column

Čepeljnik, Tadej (author), Zorec, Maša (author), Nekrep, Franc Viktor (author), Marinšek-Logar, Romana (author)

The xylanolitically active enzymes are of great interest for the industry and as feed additives as well. Therefore an effort was made to search for the microbial strains capable to degrade xylan. One of the most active rumen bacteria was Butyrivibrio sp. strain Mz5 possessing multiple xylanolytic enzyme system. In the present work the procedure for the separation of two of them is outlined. This was successfully done by anion exchange chromatography followed by gel filtration. Using the CIM DEAE tube was the key isolating point that prevented othewise frequent aggregation of the proteins and speededup the procedure. The isolated enzymes were of 51 and 58 kDa.

• Language: English
• Keywords: mikrobiologija, bakterije, Butyrivibrio, endoksilanaze, izolacija, analitska kemija, kromatografija
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publisher: Slovensko kemijsko društvo
• Year: 2002
• Number of pages: Str. 401-408
• Numbering: Letn. 49, št. 2
• PID: 20.500.12556/RUL-62869
• UDC: 579
• ISSN on article: 1318-0207
• COBISS.SI-ID: 1251720
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Acta chimica slovenica
• Shortened title: Acta chim. slov.
• Publisher: Slovensko kemijsko društvo, =Slovenian Chemical Society
• ISSN: 1318-0207
• COBISS.SI-ID: 14086149

Secondary language

• Language: Slovenian
• Title: Izolacija endoksilanaz anaerobne bakterije Buyrivibrio sp. Sev Mz5 je mogoča z uporabo anionske izmenjevalne kromatografije s CIM DEAE-8 monolitnim modulom

Abstract

Ksilanolitični encimi so pomembni v industriji celuloze in papirja ter prehranski industriji, uporabljamo pa jih tudi kot dodatek krmi ali hrani. Veliko truda je bilo vloženega v iskanje mikroorganizmov, ki so sposobni razgradnje ksilana. Ena izmed najbolj ksilanolitično aktivnih vampnih bakterij je Butyrivibrio sp. sev Mz5, ki sintetizira več različnih ksilanaz. V predstavljenem delu je opisan postopek izolacije dveh izmed njih z anionsko izmenjevalno kromtografijo in nadaljno gelsko filtracijo. Ključni izolacijski postopek je bila uporaba CIM DEAE monolitnega modula, s čimer smo se izognili drugače pogosti agregaciji proteinov in tudi pospešili celoten postopek. Izolorana encima imata molekulsko težo 51 in 58 kDa.