20.500.12556/RUL-62869 Isolation of endoxylanases from anaerobic bacterium Butyrivibrio sp. strain Mz5 is possible by anion exchange chromatography on CIM DEAE-8 monolythic column Izolacija endoksilanaz anaerobne bakterije Buyrivibrio sp. Sev Mz5 je mogoča z uporabo anionske izmenjevalne kromatografije s CIM DEAE-8 monolitnim modulom The xylanolitically active enzymes are of great interest for the industry and as feed additives as well. Therefore an effort was made to search for the microbial strains capable to degrade xylan. One of the most active rumen bacteria was Butyrivibrio sp. strain Mz5 possessing multiple xylanolytic enzyme system. In the present work the procedure for the separation of two of them is outlined. This was successfully done by anion exchange chromatography followed by gel filtration. Using the CIM DEAE tube was the key isolating point that prevented othewise frequent aggregation of the proteins and speededup the procedure. The isolated enzymes were of 51 and 58 kDa. Ksilanolitični encimi so pomembni v industriji celuloze in papirja ter prehranski industriji, uporabljamo pa jih tudi kot dodatek krmi ali hrani. Veliko truda je bilo vloženega v iskanje mikroorganizmov, ki so sposobni razgradnje ksilana. Ena izmed najbolj ksilanolitično aktivnih vampnih bakterij je Butyrivibrio sp. sev Mz5, ki sintetizira več različnih ksilanaz. V predstavljenem delu je opisan postopek izolacije dveh izmed njih z anionsko izmenjevalno kromtografijo in nadaljno gelsko filtracijo. Ključni izolacijski postopek je bila uporaba CIM DEAE monolitnega modula, s čimer smo se izognili drugače pogosti agregaciji proteinov in tudi pospešili celoten postopek. Izolorana encima imata molekulsko težo 51 in 58 kDa. mikrobiologija bakterije Butyrivibrio endoksilanaze izolacija analitska kemija kromatografija true false false Slovensko kemijsko društvo Angleški jezik Slovenski jezik Delo ni kategorizirano 2015-07-10 19:25:59 2015-07-10 19:25:59 2022-07-06 03:46:14 0000-00-00 00:00:00 2002 0 0 Str. 401-408 št. 2 Letn. 49 2002 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1318-0207 1251720 14086149 http://www.dlib.si/details/URN:NBN:SI:doc-B7BKQF8K 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=62880 Biotehniška fakulteta 0 0 0