Vaš brskalnik ne omogoča JavaScript!
JavaScript je nujen za pravilno delovanje teh spletnih strani. Omogočite JavaScript ali pa uporabite sodobnejši brskalnik.
Nacionalni portal odprte znanosti
Odprta znanost
DiKUL
slv
|
eng
Iskanje
Brskanje
Novo v RUL
Kaj je RUL
V številkah
Pomoč
Prijava
Perforin activity at membranes leads to invaginations and vesicle formation
ID
Praper, Tilen
(
Avtor
),
ID
Kladnik, Aleš
(
Avtor
),
ID
Anderluh, Gregor
(
Avtor
)
URL - Predstavitvena datoteka, za dostop obiščite
http://dx.doi.org/10.1073/pnas.1107473108
Galerija slik
Izvleček
The cytotoxic cell granule secretory pathway is essential for immune defence. How the pore-forming protein perforin (PFN) facilitates the cytosolic delivery of granule-associated proteases (granzymes) remains enigmatic. Here we show that PFN is able to induce invaginations and formation of complete internal vesicles in giant unilamellar vesicles. Formation of internal vesicles depends on native PFN and calcium and antibody labeling shows the localization of PFN at the invaginations. This vesiculation is recapitulated in large unilamellar vesicles and in this case PFN oligomers can be seen associated with the necks of the invaginations. Capacitance measurements show PFN is able to increase a planar lipid membrane surface area in the absence of pore formation, in agreement with the ability to induce invaginations. Finally, addition of PFN to Jurkat cells causes the formation of internal vesicles prior to pore formation. PFN is capable of triggering an endocytosis-like event in addition to pore formation, suggesting a new paradigm for its role in delivering apoptosis-inducing granzymes into target cells.
Jezik:
Angleški jezik
Ključne besede:
perforin
,
mambranes (biology)
,
vesicles
Vrsta gradiva:
Delo ni kategorizirano
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Leto izida:
2011
Št. strani:
Str. 21016-21021
Številčenje:
Vol. 108, no. 52
PID:
20.500.12556/RUL-37553
UDK:
577
ISSN pri članku:
0027-8424
DOI:
10.1073/pnas.1107473108
COBISS.SI-ID:
2493519
Datum objave v RUL:
10.07.2015
Število ogledov:
2467
Število prenosov:
332
Metapodatki:
Citiraj gradivo
Navadno besedilo
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Objavi na:
Gradivo je del revije
Naslov:
Proceedings of the National Academy of Sciences of the United States of America
Skrajšan naslov:
Proc. Natl. Acad. Sci. U. S. A.
Založnik:
National Academy of Sciences
ISSN:
0027-8424
COBISS.SI-ID:
286487
Podobna dela
Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:
Nazaj