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Genetic differentiation of European grayling (Thymallus thymallus) populations in Serbia, based on mitochondrial and nuclear DNA analyses
Marić, Saša (Avtor), Razpet, Andrej (Avtor), Nikolić, Vera (Avtor), Simonović, Predrag (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.gsejournal.org/content/pdf/1297-9686-43-2.pdf Novo okno

Izvleček
The structure and diversity of grayling (Thymallus thymallus) populations have been well studied in most of its native habitat; however the southernmost populations of the Balkan Peninsula remain largely unexplored. The purpose of this study was to access the genetic diversity of Serbian grayling populations, detect the impact of stocking and provide guidelines for conservation and management. Eighty grayling individuals were collected from four rivers (Ibar, Lim, Drina and Rzav). The mitochondrial DNA control region (CR; 595 bp of the 3'end and 74 bp of flanking tRNA) and the ATP6 gene (630 bp fragment) were sequenced for 20 individuals (five from each locality). In addition, all individuals were genotyped with 12 microsatellite loci. The diversity and structure of the populations as well as the recent and ancient population declines were studied using specialized software. We detected three new haplotypes in the mtDNA CR and four haplotypes in the ATP6 gene of which three had not been described before. Previously, one CR haplotype and two ATP6 gene haplotypes had been identified as allochthonous, originating from Slovenia. Reconstruction of phylogenetic relations plased the remaining two CR haplotypes from the River Danube drainage of Serbia into a new clade, which is related to the previously described sister Slovenian clade. These two clades a new Balkan clade. Microsatellite marker analysis showed that all four populations are genetically distinct from each other without any sign of intrapopulation structure, although stocking of the most diverse population (Drina River) was confirmed by mtDNA analysis. recent and historical population declines of Serbian grayling do not differ from those of other European populations. Our study shows that (1) the Ibar, Lim and Drina Rivers grayling populations are genetically distinct from populations outside of Serbia and thus should be managed as native populations in spite of some introgression in the Drina River population and (2) the Rzav River population is not appropritae for further atocking activities since it originates from stocked Slovenian grayling. However, the Rzav River population does not represent an immediate danger to other populations because it is physically isolated from these.

Jezik:Angleški jezik
Ključne besede:molekularna genetika, ribe, lipan, Thymallus thymallus, DNK, genetska diferenciacija, Srbija
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Leto izida:2011
Št. strani:str. 1-11
Številčenje:Vol. 43, no. 2
UDK:575
ISSN pri članku:1297-9686
COBISS.SI-ID:2821000 Povezava se odpre v novem oknu
Število ogledov:459
Število prenosov:155
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Gradivo je del revije

Naslov:Genetics selection evolution
Skrajšan naslov:Genet Sel Evol
Založnik:EDP sciences
ISSN:1297-9686
COBISS.SI-ID:3183636 Novo okno

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