izpis_h1_title_alt

Molecular profiling and identification of methanogenic archaeal species from rabbit caecum
Kušar, Darja (Avtor), Avguštin, Gorazd (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2010.00980.x/abstract Povezava se odpre v novem oknu

Izvleček
During a comparison of 16S rDNA PCR-denaturant gradient gel electrophoresis (DGGE) profiles of methanogenic archea from rumen fluid, rabbit caecum and pig feces, a unique band common to all rabbit caecum samples was observed. DGGE profiling also showed that the methanogen community from the New Zealand White adult rabbits is different and less complex than the methanogen communities from the rumen and pig feces. Small subunit ribosomal gene sequences of methanogenic archaea were subsequently retrieved from the constructed rabbit caecum 16S rDNA gene library. Results of the phylogenetic analysis indicated that rabbit caecum is inhibited by members of the genus Methanobrevibacter and is possibly one-species dominanted, because all the retrived sequences exhibited similarity values of 99% or higher. This species may well be a novel species of the genus Methanorevibacter. It belongs to a distinct phylogenetic group containing methanobrevibacter woesei, Methanobrevibacter thaueri and Methanobrevibacter gottschalkii strains isolated from animal faeces, and Methanobrevibactersmithii from the predominanting methanogen population of the human large bowel.

Jezik:Angleški jezik
Ključne besede:mikrobiologija, bakterije, arheje, metanogeneza, kunci, cekum, molekularna genetika, identifikacija
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Leto izida:2010
Št. strani:str. 623-630
Številčenje:Vol. 74, issue 3
UDK:579
ISSN pri članku:0168-6496
DOI:10.1111/j.1574-6941.2010.00980.x Povezava se odpre v novem oknu
COBISS.SI-ID:2744712 Povezava se odpre v novem oknu
Število ogledov:454
Število prenosov:188
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:FEMS microbiology, ecology
Skrajšan naslov:FEMS microbiol. ecol.
Založnik:Elsevier
ISSN:0168-6496
COBISS.SI-ID:6301447 Povezava se odpre v novem oknu

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