Vaš brskalnik ne omogoča JavaScript!
JavaScript je nujen za pravilno delovanje teh spletnih strani. Omogočite JavaScript ali pa uporabite sodobnejši brskalnik.
Nacionalni portal odprte znanosti
Odprta znanost
DiKUL
slv
|
eng
Iskanje
Brskanje
Novo v RUL
Kaj je RUL
V številkah
Pomoč
Prijava
Mycoplasma gallisepticum and Mycoplasma synoviae express a cysteine protease CysP, which can cleave chicken IgG into Fab and Fc
ID
Cizelj, Ivanka
(
Avtor
),
ID
Berčič, Rebeka Lucijana
(
Avtor
),
ID
Dušanić, Daliborka
(
Avtor
),
ID
Narat, Mojca
(
Avtor
),
ID
Kos, Janko
(
Avtor
),
ID
Dovč, Peter
(
Avtor
),
ID
Benčina, Dušan
(
Avtor
)
URL - Predstavitvena datoteka, za dostop obiščite
http://mic.sgmjournals.org/cgi/content/abstract/157/2/362?maxtoshow=&hits=10&RESULTFORMAT=&author1=CIyelj+&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
Galerija slik
Izvleček
Major poultry pathogens M. gallisepticum and M. synoviae share a gene encoding a putative cysteine protease CysP similar to papain cysteine (CaA subfamily). Comparison of the cysP gene sequences of 18 M. synoviae and 10 M. gallisepticum strains sequenced in this study showed polymorphisms, including deletions. Seven M. synoviae strains, including the type strain WVU 1853, had a 39 bp deletion in the 3' end of the cysP gene. In the same cysP region, all M. gallisepticum strains showed a deletion of 66bp. Immunoblot analysis with specific antibpodies demonstrated that M. synoviae strains expressed CysP, which was approximately 65 kDa. both M. synoviae and M. gallisepticum were able to digest chicken IgG (cIgG). Incubation of cIgG (-170 kDa) with M. synoviae or M. gallisepticum cells (-15 h at 37oC) resultes in a papain-like cleavage pattern of cIgG and fragments corresponding to the antigenbinding fragment of IgG (Fab, -45kDa) and the crystallizable region fragment (Fc) of the IgG heavy chain (dimer of -60 kDa). Iodoacetamide (50 mM) prevented cleavage of cIgG by both Mycoplasma species. Following site-directed mutagenesis (eight TGA codons were changed to TGG) the cysP gene of M. synoviae ULB 925 was expressed as a His-taggede protein in a cell-free system. Purified recombinant CysP (rCysp; -67kDa, pI-8) cleaved cIgG into Fab and Fc fragments. This indicates that CysP is responsible for the cIgG cleavage caused by M. synoviae and probbaly, by M. gallisepticum. This is the first evidence to our knowledge that mycopasmas have enzymes that can cleave the host IgG and indicates a novel strategy used by M. gallisepticum and M. synoviae for prolomged survival despite the antibody response of their host.
Jezik:
Angleški jezik
Ključne besede:
mikrobiologija
,
mikoplazme
,
Mycoplasma synoviae
,
Mycoplasma gallisepticum
,
encimi
,
cistin
,
proteaze
Vrsta gradiva:
Delo ni kategorizirano
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Leto izida:
2011
Št. strani:
Str. 362-372
Številčenje:
Vol. 157, no. 2
PID:
20.500.12556/RUL-36535
UDK:
579
ISSN pri članku:
1350-0872
DOI:
10.1099/mic.0.045641-0
COBISS.SI-ID:
2835848
Datum objave v RUL:
10.07.2015
Število ogledov:
2284
Število prenosov:
330
Metapodatki:
Citiraj gradivo
Navadno besedilo
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Objavi na:
Gradivo je del revije
Naslov:
Microbiology
Skrajšan naslov:
Microbiol
Založnik:
Society for General Microbiology
ISSN:
1350-0872
COBISS.SI-ID:
1176084
Podobna dela
Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:
Nazaj