We present a set of nuclear DNA SNPs enabling discrimination between Salmo marmoratus, S. obtusirostris and different evolutionary lineages of S. trutta. The loci containing SNPs were either isolated from marble trout nuclear DNA or inferred from literature. By comparing the joint sequences (ca. 5,830 bp) among 48 tested individuals, 127 substitutions and 8 indels were found. Forty-one of them, distributed across 16 loci, turned out as species orlineages specific. This set of SNP markers may be applied as molecular tool for genetic management purposes as well as in revolving phylogenetic and taxonomic uncertainties of different trout stocks.