The neuronal splicing factor Nova co-localizes with target RNAs in the dendrite

Racca, Claudia (avtor), Ule, Jernej (avtor)

Nova proteins are neuron-specifi c RNA binding proteins targeted by autoantibodies in a disorder manifest by failure of motor inhibition, and they regulate splicing and alternative 3 processing. Nova regulates splicing of RNAs encoding synaptic proteins, including the inhibitory glycine receptor f 2 subunit (GlyRf 2), and binds to others, including the GIRK2 channel. We found that Nova harbors functional NES and NLS elements, shuttles between the nucleus and cytoplasm, and that 50% of the protein localizes to the soma-dendritic compartment. Immunofl uoresence and EM analysis of spinal cord motor neurons demonstrated that Nova co-localizes beneath synaptic contacts indendrites with the same RNA, GlyRf 2, whose splicing it regulates in the nucleus. HITS-CLIP identifi ed intronic and 3 UTR sites where Nova binds to GlyRf 2 and GIRK2 transcripts in the brain. This led directly to the identifi cation of a 3 UTR localization element that mediates Nova-dependent localization of GIRK2 in primary neurons. These data demonstrate that HITS-CLIP can identify functional RNA localization elements, and they suggest new links between the regulation of nuclear RNA processing and mRNA localization.

• Jezik: Angleški jezik
• Ključne besede: RNK, beljakovine, nevroni, hrbtenjača
• Vrsta gradiva: Delo ni kategorizirano
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FDV - Fakulteta za družbene vede
• Leto izida: 2010
• Št. strani: Str. 1-16
• Številčenje: Vol. 4, art. 5
• PID: 20.500.12556/RUL-33303
• UDK: 577.2
• ISSN pri članku: 1662-5110
• DOI: 10.3389/neuro.04.005.2010
• COBISS.SI-ID: 30083165
• Datum objave v RUL: 10.07.2015

Gradivo je del revije

• Naslov: Frontiers in neural circuits
• Skrajšan naslov: Front. neural circuits
• Založnik: Frontiers Research Foundation
• ISSN: 1662-5110
• COBISS.SI-ID: 30082909