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The neuronal splicing factor Nova co-localizes with target RNAs in the dendrite
ID
Racca, Claudia
(
Author
),
ID
Ule, Jernej
(
Author
)
URL - Presentation file, Visit
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2856630/pdf/fncir-04-005.pdf
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Abstract
Nova proteins are neuron-specifi c RNA binding proteins targeted by autoantibodies in a disorder manifest by failure of motor inhibition, and they regulate splicing and alternative 3 processing. Nova regulates splicing of RNAs encoding synaptic proteins, including the inhibitory glycine receptor f 2 subunit (GlyRf 2), and binds to others, including the GIRK2 channel. We found that Nova harbors functional NES and NLS elements, shuttles between the nucleus and cytoplasm, and that 50% of the protein localizes to the soma-dendritic compartment. Immunofl uoresence and EM analysis of spinal cord motor neurons demonstrated that Nova co-localizes beneath synaptic contacts indendrites with the same RNA, GlyRf 2, whose splicing it regulates in the nucleus. HITS-CLIP identifi ed intronic and 3 UTR sites where Nova binds to GlyRf 2 and GIRK2 transcripts in the brain. This led directly to the identifi cation of a 3 UTR localization element that mediates Nova-dependent localization of GIRK2 in primary neurons. These data demonstrate that HITS-CLIP can identify functional RNA localization elements, and they suggest new links between the regulation of nuclear RNA processing and mRNA localization.
Language:
English
Keywords:
RNK
,
beljakovine
,
nevroni
,
hrbtenjača
Work type:
Not categorized
Typology:
1.01 - Original Scientific Article
Organization:
FDV - Faculty of Social Sciences
Year:
2010
Number of pages:
Str. 1-16
Numbering:
Vol. 4, art. 5
PID:
20.500.12556/RUL-33303
UDC:
577.2
ISSN on article:
1662-5110
DOI:
10.3389/neuro.04.005.2010
COBISS.SI-ID:
30083165
Publication date in RUL:
10.07.2015
Views:
1136
Downloads:
271
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Record is a part of a journal
Title:
Frontiers in neural circuits
Shortened title:
Front. neural circuits
Publisher:
Frontiers Research Foundation
ISSN:
1662-5110
COBISS.SI-ID:
30082909
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