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The neuronal splicing factor Nova co-localizes with target RNAs in the dendrite
ID
Racca, Claudia
(
Avtor
),
ID
Ule, Jernej
(
Avtor
)
URL - Predstavitvena datoteka, za dostop obiščite
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2856630/pdf/fncir-04-005.pdf
Galerija slik
Izvleček
Nova proteins are neuron-specifi c RNA binding proteins targeted by autoantibodies in a disorder manifest by failure of motor inhibition, and they regulate splicing and alternative 3 processing. Nova regulates splicing of RNAs encoding synaptic proteins, including the inhibitory glycine receptor f 2 subunit (GlyRf 2), and binds to others, including the GIRK2 channel. We found that Nova harbors functional NES and NLS elements, shuttles between the nucleus and cytoplasm, and that 50% of the protein localizes to the soma-dendritic compartment. Immunofl uoresence and EM analysis of spinal cord motor neurons demonstrated that Nova co-localizes beneath synaptic contacts indendrites with the same RNA, GlyRf 2, whose splicing it regulates in the nucleus. HITS-CLIP identifi ed intronic and 3 UTR sites where Nova binds to GlyRf 2 and GIRK2 transcripts in the brain. This led directly to the identifi cation of a 3 UTR localization element that mediates Nova-dependent localization of GIRK2 in primary neurons. These data demonstrate that HITS-CLIP can identify functional RNA localization elements, and they suggest new links between the regulation of nuclear RNA processing and mRNA localization.
Jezik:
Angleški jezik
Ključne besede:
RNK
,
beljakovine
,
nevroni
,
hrbtenjača
Vrsta gradiva:
Delo ni kategorizirano
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FDV - Fakulteta za družbene vede
Leto izida:
2010
Št. strani:
Str. 1-16
Številčenje:
Vol. 4, art. 5
PID:
20.500.12556/RUL-33303
UDK:
577.2
ISSN pri članku:
1662-5110
DOI:
10.3389/neuro.04.005.2010
COBISS.SI-ID:
30083165
Datum objave v RUL:
10.07.2015
Število ogledov:
1192
Število prenosov:
271
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Frontiers in neural circuits
Skrajšan naslov:
Front. neural circuits
Založnik:
Frontiers Research Foundation
ISSN:
1662-5110
COBISS.SI-ID:
30082909
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